RT Quantitative Thermal Cycler

What Is Real-Time Quantitative PCR

RQ-PCR measures PCR product accumulation through a dual-labeled fluorogenic probe (TaqMan Probe), it provides accurate and reproducible quantitation of gene copies. Unlike other quantitative PCR methods, RQ-PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. The RQl-PCR method has a very large dynamic range of starting target molecule determination. RQ-PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.