Nucleic Acid Extraction
An extractor's purpose is to disintegrate the cell envelope and achieve maximum elimination of lipids and proteins to obtain pure DNA and/or RNA. There are 3 basic steps involved in DNA extraction, that is lysis, precipitation and purification. In lysis, the nucleus and the cell are broken open, thus releasing DNA. This process involves mechanical disruption and uses enzymes and detergents like Proteinase K to dissolve the cellular proteins and free DNA.
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